Tools

Description: CD3 (Clone SK7): The CD3/T-cell receptor (TCR) complex is composed of two CD3ε, one CD3γ, one CD3Π... more

Description: CD3 (Clone SK7): The CD3/T-cell receptor (TCR) complex is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. It plays a role in antigen recognition, signal transduction, and T cell activation.CD45 (Clone 2D1): It is a type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It functions as a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation.CD56 (Clone 5.1H11): A single transmembrane glycoprotein, also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is expressed on NK and NKT cells, but in other cells and tissues as well, such as large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, myeloid leukemias, brain cerebellum and cortex, and neuromuscular junctions. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.CD16 (Clone B73.1): Also known as low affinity IgG receptor III (FcγRIII), it is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells, and placental trophoblasts in humans. CD16b (FcγRIIIB) is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).CD19 (Clone SJ25C1): Also known as B4, it is a member of the immunoglobulin superfamily expressed on B cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.   collapse

Description: In mice, CD4+ T cells can be divided into naïve and memory cells based on expression of adhesion mo ... more

Description: In mice, CD4+ T cells can be divided into naïve and memory cells based on expression of adhesion molecules CD44 and CD62L. Naïve T cells exhibit high levels of CD62L and low expression of CD44, whereas memory T cells are identified by high CD44 and low CD62L expression. This panel includes antibodies against CD3, CD4, CD62L and CD44, which can be used together to identify naïve and memory T cell subsets in mice. Alterations in these subsets are evident in older mice following antigenic stimulation.   collapse

Description: CD3: SK7 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell rece ... more

Description: CD3: SK7 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD4: SK3 antibody reacts with CD4, a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD45: HI30 antibody reacts with CD45, a 180-240 kD single chain type I membrane glycoprotein which is also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets.   collapse

Description: This anti-Human Lineage Cocktail is optimized for the detection of human lymphocytes, monocytes, eos ... more

Description: This anti-Human Lineage Cocktail is optimized for the detection of human lymphocytes, monocytes, eosinophils, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells (DCs), including DCs that express CD16. This cocktail is composed of CD3, CD14, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD19 and CD20 are on B cells; CD56 is located on NK cells.   collapse

Description: FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IP ... more

Description: FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.   collapse