Assay kits

Description: The Loading Control Antibody Sampler Kit contains antibodies to a variety of housekeeping proteins. ... more

Description: The Loading Control Antibody Sampler Kit contains antibodies to a variety of housekeeping proteins. Loading controls are essential for the interpretation of western blot data. Loading control antibodies allow the verification of equal protein loading between samples. Ideal loading controls are expressed constitutively and at high levels with low variability between cell lines and experimental conditions. Also, the loading control should be at a different molecular weight than the protein of interest this allows the bands to be visually distinguishable. The Loading Control Antibody Sampler kit contains enough primary and secondary antibodies to perform ten western blots per loading control antibody.   collapse

Description: The kit provides a means of performing enzymatic assays with active human ABL1 kinase. It includes a ... more

Description: The kit provides a means of performing enzymatic assays with active human ABL1 kinase. It includes active ABL1 kinase (supplied as a GST fusion protein), a biotinylated substrate peptide and a phospho-tyorsine monoclonal antibody for detection of the phosphorylated form of the substrate peptide. Molecular Formula: Peptide Substrate, Biotin-Signal Transduction Protein (Tyr160): 1830 Daltons, GST-ABL1 Kinase domain: 74,140 Daltons Peptide Core Sequence: GIY*DV   collapse

Description: Use Rockland's MaxTag™ Kit for Duplex IRDYE® Immunoblotting for the detection of Rabbit and Mouse pr ... more

Description: Use Rockland's MaxTag™ Kit for Duplex IRDYE® Immunoblotting for the detection of Rabbit and Mouse primary antibodies using infrared-labeled antibodies to form an antigen-antibody-antibody complex that is immobilized on a membrane (immunoblot) and detected using a two channel infrared scanner such as LI-COR's Odyssey® Infrared Imaging System. This Kit is useful for both “western blotting” and “dot blotting” methods and can be used for single (1-color) or duplex (2-color) antibody detection (see Figure 3). Nitrocellulose membrane formats are preferred (see sections XI and XII for more details). If you require additional assistance please call or e-mail our technical service representatives at 800-656-7625 or tech@rockland-inc.com.   collapse

Description: The Zombie dyes in this kit (provided at 100 tests each) are the UV, NIR, Violet, Aqua and Yellow va ... more

Description: The Zombie dyes in this kit (provided at 100 tests each) are the UV, NIR, Violet, Aqua and Yellow variants. The Zombie live/dead fixable dyes are amine-reactive fluorescent dyes that are impermeant to live cells but permeant to cells with compromised membranes. They irreversibly conjugate to primary amine-containing proteins. Since there is a greater abundance of proteins inside the cell versus the cell surface, dead cells with a compromised membrane stain brighter than live cells with an intact membrane. Thus, they can be used to assess live vs. dead status of mammalian cells. However, titration of the reagent is required to ensure that live cells have minimal to no staining due to this dye on the cell type of interest. It is also important to consider that the accuracy of the live/dead assessment is dependent on the uniformity in size of each cell since larger cells will stain brighter than smaller cells, possibly confusing large cells as dead cells. Zombie live/dead fixable probes are useful for incorporation into multicolor panels, particularly in applications where cells will be fixed and permeabilized prior to analysis. To find the spectra of each of the Zombie dyes offered in this kit, please find them here:  https://www.biolegend.com/spectraanalyzer   collapse

Description: The kit provides a means of performing kinase activity assays with recombinant human Abl1 T315I kina ... more

Description: The kit provides a means of performing kinase activity assays with recombinant human Abl1 T315I kinase. It includes active Abl1 T315I kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine monoclonal antibody for detection of the phosphorylated form of the substrate peptide. Molecular Formula: Peptide substrate, Biotin-Signal Transduction Protein (Tyr160): 1830 Daltons. GST-Abl1 T315I Kinase: 76 kDa. Peptide Core Sequence: GIY*DV   collapse