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Author: Dr. Meindert Lamers https://www.lumc.nl/org/chemische-immunologie/onderzoek/LamersLab1/ Preparation of Biotin PEG slides Day 1 1. Labeling of the slides Label around 120 22x22mm slides: scratch 3 to the upper right corner Material: Slides: 22x22mm VWR International Thickness 1 2. Wash the slides Put the slides into a 3M NaOH solution in a glass jar on Teflon rack, sonicate them for 35-40min. In the meantime, do the following Wash the weighing jars with water, then with 3M NaOH, .... (Download Document)

Search by: Same CST. New Website. Learn more Register  // Sign In  // Cart (0 items) Are you in Industry or Academia? Search .... (Download Document)

Home Join Contact About Us Open Access Support Us News & Events OMICS Journals Call for Proposals Submit Manuscript Search   Research Article Open Access An Effective Method for the Analysis of Human Plasma Proteome using Two-dimensional Gel Electrophoresis Yasmin Ahmad1* and Narendra Sharma1 1Peptide and Proteomics, Defence Institute of Physiology & Allied Scie .... (Download Document)

FACS analysis using splenocytes 1. Introduction Fluorescence activated cell sorter (FACS) is a powerful tool to measure and analyze cell surface molecules of single cells which flow in fluid stream through a beam of light to detect the fluorescences of the cells. Animal experiments for biomedical research are required using the animals of assured genetic quality. FACS can be applied to determine immunological profiling of the various mouse strains such as standard inbred and immuno .... (Download Document)

For research use only. Not for diagnostic or therapeutic applications. 24 Whitewoods Lane, Malvern, PA 19355 Tel: (610) 945-2007 FAX: (610) 945-2008 E-mail: sale@neweastbio.com Web: www.neweastbio.com Protocol for IF/IHC 1. Rinse cells briefly in PBS (37℃, as quickly as possible). 2. Aspirate PBS, cover cells with 300~500 µl 4% formaldehyde in PBS. 3. Allow cells to fix for 15 minutes at room temperature. 4. Aspirate fixative, rinse three times in PBS for 1 minute .... (Download Document)

Protocols Direct ELISA Required Reagents: Antigen (preferably purified) HRP-Conjugated Primary Antibody Coating Buffer, 0.05 M Carbonate-Bicarbonate, pH 9.6 Wash Solution, 50 mM Tris, 0.14 M NaCl, 0.05% Tween 20, pH 8.0 Blocking (Postcoat) Solution, 50 mM Tris, 0.14 M NaCl, 1% BSA, pH 8.0 Conjugate Diluent, 50 mM Tris, 0.14 M NaCl, 1% BSA, 0.05% Tween 20, pH 8.0 Enzyme Substrate, TMB Stop Solution, 0.18 M H2SO4 or other appropriate solution High Protein Bindi .... (Download Document)

Protocol for ELISA Kit from Biorbyt: Tools for Science FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. Human TGFβ1 ELISA Kit Preparation  Plate Washing Discard the solution in the plate without touching the side walls. Blot the plate onto paper towels or other absorbent material. Soak each well with at least 0.3 ml PBS or TBS buffer for 1~2 minutes. Repeat this process two additional times for a total of THREE washes. Note: For automated washing, aspirate all wells .... (Download Document)

1 Cell Migration, Chemotaxis and Invasion Introduction Cell migration, the movement of cells from one area to another generally in response to a chemical signal, is central to achieving functions such as wound repair, cell differentiation, embryonic development and the metastasis of tumors. Cell invasion is similar to cell migration; however, it requires a cell to migrate through an extracellular matrix (ECM) or basement membrane extract (BME barrier by first enzyma .... (Download Document)

Home | Jobs | News & Articles | Job Advice | Search | Protocols | Fun | @RealLabRat Candidates - post your resume Candidates - search biotech jobs Employers - post job openings Email Login Password New users sign up! HOME > Protocols > Histology > Staining protocols > Protocol for Immunopero .... (Download Document)

Low cell‐number Native Chromatin  Immunoprecipitation‐sequencing (ChIP‐seq) Protocol  Supplementary method: Gilfillan et al.    The protocol below is written for frozen, live cells, but can easily be adapted for use with freshly  prepared samples. As written below, it has been optimised for sorted CD4+ & CD8+ lymphocytes in  the range of 2 x 105 – 1 x 106 cells (starting cell numbers). For lower cell numbers, scaling all volumes  down by a factor of 5 has been tested and works well down to at .... (Download Document)

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