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1 Product Code: HTI006 Number of experiments that can be performed: 5/20 Duration of Experiment: 2 days Day1- Protocol: 2 hours Day2- Observations: 15 minutes Storage Instructions: � The kit is stable for 6 months from the date of receipt � Store Antiserum, Standard and Test Antigens at 2-8oC � Other kit contents can be stored at room temperature (15-25oC) HiPer® Rocket Immunoelectrophores .... (Download Document)

A. Solutions and Reagents 1. 1X SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red. 2. Transfer Buffer: 25 mM Tris base, 0.2 M glycine, 20% methanol (pH 8.5). 3. 10X Tris Buffered Saline (TBS): To prepare 1 liter of 10X TBS: 24.2 g Tris base, 80 g NaCl; adjust pH to 7.6 with HCl (use at 1X). 4. Nonfat Dry Milk: (weight to volume [w/v]). 5. Blocking Buffer: 1X TBS, 0.1% Tween-20 with 5% w/v nonfat .... (Download Document)

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H Products ▼ Flash Chromatography SINGLE StEP® Columns SINGLE StEP® Standard Silica SINGLE StEP® E. Merck Silica SINGLE StEP® C18 SINGLE StEP® Amine SINGLE StEP® Cyano SINGLE StEP® Diol SINGLE StEP® Phenyl SINGLE StEP® Empty Columns Syringe Barrel Columns Standard Silica E. Merck Silica Empty Syringe Columns Bulk Sorbent Head Space Frits Accessories & Adapters Technical Lib .... (Download Document)

  Home  ■  Participation  ■  Assessments  ■  Epitopes  ■  Protocols  ■  Techniques  ■  Links Recommended p53 protocols  ■  Recommended p53 control tissue Assessment Run 20 2007   p53 The slide to be stained for p53 comprised: 1. Liver, .... (Download Document)

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Protocol for ELISA Kit from Biorbyt: Tools for Science FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. Rat PDGF-AB ELISA Kit Preparation  Plate Washing Discard the solution in the plate without touching the side walls. Blot the plate onto paper towels or other absorbent material. Soak each well with at least 0.4 ml PBS or TBS buffer for 1~2 minutes. Repeat this process two additional times for a total of THREE washes. Note: For automated washing, aspirate all wells .... (Download Document)

pdfcrowd.comopen in browser PRO version Are you a developer? Try out the HTML to PDF API Home Molecular biology Cell biology Neuroscience Biochemistry Immunology Antibody BIOLOGY ASSAYS & PROTOCOLS Immunoprecipitation (IP) protocol Co-Immunoprecipitation (Co-IP) Chromatin immunoprecipitation (ChIP) protocol Yeast transformation protocol SDS PAGE Protocol Co-IP Protocol Western Blot Protocol ELISA Protocol H7N9 HA/Hemagglutinin (New) Co-Immunoprecipitation (Co-IP) Background&Protocol Co-Im .... (Download Document)

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