The intelligent search engine for Life Sciences

Paraffin embedding and sectioning From OpenWetWare Jump to: navigation, search     back to protocols Biological samples often need to be solidified to allow fine sectioning. Thin slices improve the access of dyes, probes, and antibodies and reduce the overlay of different cells layers in the z direction. For light microscopy, paraffin wax is the most frequently used hard matrix for cutting. Contents [hide] 1 Principle 2 Secti .... (Download Document)

Protocol for ELISA Kit from Biorbyt: Tools for Science FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. Mouse IL-1α ELISA Kit Preparation  Plate Washing Discard the solution in the plate without touching the side walls. Blot the plate onto paper towels or other absorbent material. Soak each well with at least 0.3 ml PBS or TBS buffer for 1~2 minutes. Repeat this process two additional times for a total of THREE washes. Note: For automated washing, aspirate all wells .... (Download Document)

Protocol for ELISA Kit from Biorbyt: Tools for Science FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. Mouse M-CSF ELISA Kit Preparation  Plate Washing Discard the solution in the plate without touching the side walls. Blot the plate onto paper towels or other absorbent material. Soak each well with at least 0.3 ml PBS or TBS buffer for 1~2 minutes. Repeat this process two additional times for a total of THREE washes. Note: For automated washing, aspirate all wells .... (Download Document)

MATERIAL SAFETY DATA SHEET Cat# Orb61888, Pimecrolimus PAGE 1 OF 4 SECTION 1: PRODUCT AND COMPANY IDENTIFICATION PRODUCT NAME: Pimecrolimus Catalog number: Orb61888 Company: Biobyt Ltd. ADDRESS: 5 Orwell Furlong, Cowley Road Cambridge, Cambridgeshire .... (Download Document)

Elisa Protocol Materials:   ·         96-Well Microtiter Plates ·         Eppendorf Tubes ·         Twelve-Channel Pipettor ·         1mL Adjustable Pipettor ·         Humid Chamber ·         Wash Bottle or ELISA Plate Washer ·         ELISA Plate Reader ·         TMB   Buffer Formulations:   Carbonate Buffer 1 Liter – pH 9.5 – Store at 4°C ·         1.59g Na2CO3 ·         2.93g NaHCO3 ·         2mL 10% NaN3 ·         DI H2O to 1L   Wash Buffer · .... (Download Document)

General Matrigel Angiogenesis Assay Each animal will have two Matrigel implants of 250ul each. One with Matrigel/heparin and the other with Matrigel/heparin/bFGF Matrigel Preparation/Injection: Materials needed - 1 vial Matrigel 10ml at ~15mg/ml stock, Low Endotoxin! Some batches have killed mice due to high endotoxin levels! Prechilled Falcon tubes and 1ml pipet tips Prechilled tuberculin syringes with removable needles 27G needles for injection Avertin - 500ul/animal ~ 50ml 3CC needles for .... (Download Document)

Home About Help FAQ Blog Support Register Tour Contact eLabJournal - Laboratory Information Management System Login .... (Download Document)

Protocol for ELISA Kit from Biorbyt: Tools for Science FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. Human MMP-3 ELISA Kit Preparation  Plate Washing Discard the solution in the plate without touching the side walls. Blot the plate onto paper towels or other absorbent material. Soak each well with at least 0.3 ml PBS or TBS buffer for 1~2 minutes. Repeat this process two additional times for a total of THREE washes. Note: For automated washing, aspirate all wells .... (Download Document)

Discover more at abcam.com/technical Nuclear Fractionation Protocol Reagents Buffer A 10 mM HEPES, 1.5 mM MgCl2, 10 mM KCl, 0.5 mM DTT, 0.05% NP40 (or 0.05% Igepal or Tergitol) pH 7.9 To prepare 250 ml stock of buffer A – HEPES: 1M = 238.3 g/L, therefore 10 mM = 0.59 g/250ml MgCl2: 1M = 203.3 g/L, therefore 1.5 mM = 0.076 g/250ml KCl: 1M = 74.5 g/L, therefore 10 mM = 0.187 g/250ml DTT: 1M = 154.2 g/L, therefore 0.5 mM = 0.019 g/250ml NP40 = 0.05% Buffer B 5 mM HE .... (Download Document)

SDS-PAGE (Laemmli) Casting and running protein gels according to Laemmli using BioRad’s Minipro- tean II. The protocol 1. Take two spacers, a comb, one small and one large glass plate, a casting block and the casting stand. 2. Clean the glass plates with ethanol. 3. Assemble the “sandwich”, take case that the lower edges of blass plates and spacers are well aligned. Otherwise the whole thing will leak when you pour the gel. 4. Prepare 10 % (w/v) ammoniumpersulfate (APS) solution fresh 5. .... (Download Document)

1520 results found in 29 ms (Page 2 of 152)