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Protocol for ELISA Kit from Biorbyt: Tools for Science FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. Human IL-3 ELISA Kit Preparation  Plate Washing Discard the solution in the plate without touching the side walls. Blot the plate onto paper towels or other absorbent material. Soak each well with at least 0.3 ml PBS or TBS buffer for 1~2 minutes. Repeat this process two additional times for a total of THREE washes. Note: For automated washing, aspirate all wells .... (Download Document)

Polymerase Chain Reaction Protocol Overview This is a standard PCR protocol used on all first pass (unoptimized) PCR amplifications. This protocol outlines: Setup of a single PCR reaction Preparation of PCR-ready 96-well plates with Elongase MasterMix PCR setup for amplification We have tested numerous PCR reagent kits from different vendors (e.g., AmpliTaq (Perkin-Elmer)) to identify a kit which produces simple, robust, and very specific reactions, and allows for m .... (Download Document)

IMMUNOFLUORESCENCE All Hycult Biotech products are subject to strict quality control procedures. Copyright® Hycult Biotech. All rights reserved. The information on this sheet should neither be considered comprehensive or definitive. Website:www.hycultbiotech.com pag 1 of 3 Version: 04-2010 Introduction Immunofluorescence is the labeling of antigens with fluorescent dyes. This technique is often used to visualize subcellular distribution of biomolecules of interest. Immunofluoresc .... (Download Document)

Protocol for ELISA Kit from Biorbyt: Tools for Science FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. Human IL-12(p70) ELISA Kit Preparation  Plate Washing Discard the solution in the plate without touching the side walls. Blot the plate onto paper towels or other absorbent material. Soak each well with at least 0.3 ml PBS or TBS buffer for 1~2 minutes. Repeat this process two additional times for a total of THREE washes. Note: For automated washing, aspirate all .... (Download Document)

Protocol For FFPE tissues 1. H&E stain 1/8 2. Immunohistochemical stain 2/8 For Frozen tissues 1. H&E stain 5/8 2. Immunohistochemical stain 6/8 Carefully open the slide case H&E Staining Protocol for formalin fixed paraffin embedded (FFPE) tissues For research use only 11. Drop 2-3 drops of mountant onto the slide and, then, put a cover glass onto the slide •Mounting 10. Dip the rack into the fo .... (Download Document)

Protocol for ELISA Kit from Biorbyt: Tools for Science FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. Mouse IGF-1 ELISA Kit Preparation  Plate Washing Discard the solution in the plate without touching the side walls. Blot the plate onto paper towels or other absorbent material. Soak each well with at least 0.3 ml PBS or TBS buffer for 1~2 minutes. Repeat this process two additional times for a total of THREE washes. Note: For automated washing, aspirate all wells .... (Download Document)

SANTA CRUZ BIOTECHNOLOGY, INC. Fibronectin: sc-29011 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved i .... (Download Document)

Protocol for ELISA Kit from Biorbyt: Tools for Science FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. Rat TNFα ELISA Kit Preparation  Plate Washing Discard the solution in the plate without touching the side walls. Blot the plate onto paper towels or other absorbent material. Soak each well with at least 0.3 ml PBS or TBS buffer for 1~2 minutes. Repeat this process two additional times for a total of THREE washes. Note: For automated washing, aspirate all wells an .... (Download Document)

StressMarq Biosciences Ince 1 250 294 9064 info@stressmarq com StressMarq Biosciences Inc. IMMUNOHISTOCHEMISTRY (IHC) - PROTOCOL Principle Immunohistochemistry staining is used to identify specific constituents in tissue sections or immobilized cells. To detect the reaction site, the antibody/antigen complex is labeled with an enzyme that can be reacted with a suitable substrate to give a colored product. Proper fixation is crucial to successful staining. Formaldehyde fixation is ofte .... (Download Document)

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