RAT IgG1 isotype control Fluorescein

RAT IgG1 isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain a - IgA, ? - IgG 1, 2a, 2b, 2c and µ - IgM, light chain ? and ?.

  • Product Detail
  • All Images
Category Peptides
Price $89.00
Publication 0
Application ELISA, IF/ICC , FC
Purification RAT IgG1 isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG2a, IgG2b, IgG3 , IgM , and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed.
Application Note RAT IgG1 isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
Form Liquid (sterile filtered)
Storage Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Supplier Rockland Inc.
Catalog Number 012-002-040
Concentration 1.0 mg/mL by UV absorbance at 280 nm
Alternative Names Rat Isotype Control, Rat IgG1 FITC isotype, RAT IgG1 subclass isotype control
Size 50 µg
Biological Origin Rat

Images are not available.

Feedback