Mouse IgG2b isotype control Phycoerythrin

Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.

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Category Peptides
Price $215.00
Publication 0
Application ELISA, IF/ICC , FC
Purification This product was prepared from ascitic fluid by affinity chromatography.   Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Phycoerythrin and Anti-Mouse Serum.  Specificity was confirmed by ELISA at less than 1% cross reactivity against other anti-Mouse heavy or light chain isotypes antibodies.
Application Note Mouse IgG2b isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Form Lyophilized
Storage Store isotype control at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Supplier Rockland Inc.
Catalog Number 010-0842
Concentration 1 mg/ml by absorbance = 82.0 at 565 nm
Alternative Names Mouse IgG2b isotype control, Mouse IgG2b subclass isotype control
Size 1 mL
Biological Origin Mouse
Tissue Location 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2