DYKDDDDK (FLAG®) Peptide

The FLAG® peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys or DYKDDDDK) is intended for immunoaffinity chromatography to allow elution under non-denaturing conditions by competitive elution with excess of free FLAG® Peptide.

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Category Peptides
Price $85.00
Publication 0
Application WB, IP
Purification Greater than 90% specific peptide by HPLC and MS.
Application Note A working concentration of 500 µg/mL for triple FLAG® peptide is typically used for competitive elution of FLAG® containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG® affinity gel PROTOCOL for specific instructions for use.
Form Lyophilized
Storage Store vial at 2 - 8 ° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Supplier Rockland Inc.
Catalog Number 000-000-383
Concentration 1 mg/mL by dry weight
Alternative Names synthetic FLAG® peptide, ddddk peptide, dykddddk peptide, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, control peptide, blocking peptide
Size 1.0 mg
Tissue Location None
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