beta Actin Antibody

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins. The isotype of this antibody is mouse IgM. It can be detected with the secondary antiody "SA00001-1" Peroxidase-conjugated Affinipure Goat Anti-Mouse IgG(H+L).

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Category Antibodies
Publication 695
Application ELISA, FC, IF, IHC, WB
Host Mouse
Reactivity Human, mouse, rat, ZebraFish, pig, Yeast, arabidopsis, cabbage, nematode, soybean, A. sphaerocephala Krasch, bovine, C. tubulosa, Chicken, Drosophila, duck, eels, Eelworm, Gekko japonicus, Hamster, Rare minnows, rice, sheep
Clonality Monoclonal
Purification Caprylic acid/ammonium sulfate precipitation
immunogen beta Actin fusion protein Ag0297
Form PBS with 0.1% sodium azide and 50% glycerol pH 7.3 .
Storage Store at -20°C. Aliquoting is unnecessary for -20°C storage.
Supplier Proteintech
Isotype IgM
MW 375aa,42 kDa
Dilution WB : 1:2000-1:20000IHC : 1:50-1:500IF : 1:20-1:200
Catalog Number 60008-1-Ig
Alternative Names ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, β actin
Size 150ul
Clone 7D2C10
Gene Symbol ACTB