GAPDH Antibody

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

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Category Antibodies
Publication 567
Application ELISA, FC, IF, IHC, IP, WB
Host Mouse
Reactivity Human, mouse, rat, ZebraFish, Yeast, cow, Cyprinus carpio, Eelworm, hamster, monkey, moth, pig, Xenopuslaevis
Clonality Monoclonal
Purification Protein A purification
immunogen GAPDH fusion protein Ag0766
Form PBS with 0.1% sodium azide and 50% glycerol pH 7.3 .
Storage Store at -20°C. Aliquoting is unnecessary for -20°C storage.
Supplier Proteintech
Isotype IgG2b
MW 36 kDa
Dilution WB : 1:5000-1:20000IP : 0.5-4.0 ug for IP and 1:3000-1:20000 for WBIHC : 1:50-1:500IF : 1:20-1:200
Catalog Number 60004-1-Ig
Alternative Names G3PD, GAPD, GAPDH, OK/SW cl.12
Size 150ul
Clone 1E6D9
Gene Symbol GAPDH