Acetyl-Histone H4 (Lys5) Antibody

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1,2). Histone acetylation occurs mainly on the amino-terminal tail domains of histones H2A (Lys5), H2B (Lys5, 12, 15, and 20), H3 (Lys9, 14, 18, 23, 27, 36 and 56), and H4 (Lys5, 8, 12, and 16) and is important for the regulation of histone deposition, transcriptional activation, DNA replication, recombination, and DNA repair (1-3). Hyper-acetylation of the histone tails neutralizes the positive charge of these domains and is believed to weaken histone-DNA and nucleosome-nucleosome interactions, thereby destabilizing chromatin structure and increasing the accessibility of DNA to various DNA-binding proteins (4,5). In addition, acetylation of specific lysine residues creates docking sites for a protein module called the bromodomain, which binds to acetylated lysine residues (6). Many transcription and chromatin regulatory proteins contain bromodomains and may be recruited to gene promoters, in part, through binding of acetylated histone tails. Histone acetylation is mediated by histone acetyltransferases (HATs), such as CBP/p300, GCN5L2, PCAF, and Tip60, which are recruited to genes by DNA-bound protein factors to facilitate transcriptional activation (3). Deacetylation, which is mediated by histone deacetylases (HDAC and sirtuin proteins), reverses the effects of acetylation and generally facilitates transcriptional repression (7,8).

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Category Category: Primary Antibodies
Publication 0
Application Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry),Chromatin IP
Specificity Acetyl-Histone H4 (Lys5) Antibody detects endogenous levels of histone H4 only when acetylated on Lys5. This antibody does not cross-react with histone H4 acetylated on lysines 8, 12, or 16.
Host Rabbit
Reactivity Human, Mouse, Rat, Monkey
Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H4 in which Lys5 is acetylated. Antibodies are purified by protein A and peptide affinity chromatography.
Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100
Supplier Cell Signaling Technology
MW 11
Dilution 1:1000,1:25,1:1600,1:800,1:25
Catalog Number 9672
Size 20
ELISA Sensitivity Endogenous