B-Raf (L12G7) Mouse mAb

A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497, and Ser499 (2). p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity (3,4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6,7). While A-Raf, B-Raf, and c-Raf are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428, and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8,9). Research studies have shown that the B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301, and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events (11).

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Category Category: Primary Antibodies
Publication 0
Application Western Blotting,Immunoprecipitation,Flow Cytometry
Specificity B-Raf (9L12G7) Mouse mAb detects endogenous levels of total B-Raf protein.
Host Mouse
Reactivity Human, Mouse, Rat, Monkey, D. melanogaster
Purification Monoclonal antibody is produced by immunizing animals with a recombinant fragment of B-Raf protein.
Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100
Supplier Cell Signaling Technology
MW 86
Dilution 1:1000,1:50,1:400
Catalog Number 9434
ELISA Sensitivity Endogenous
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