PathScan

Monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser473 of human Akt, Thr202/Tyr204 of human p44 MAP kinase, and Ser235/Ser236 of human ribosomal protein S6.

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Category Category: Primary Antibodies
Publication 0
Application Primary Cocktail,Detection Cocktail,Phospho-Akt (Ser473),Phospho-p44/42 Erk1/2 (Thr202/Tyr204),Phospho-S6 Ribosomal Protein (Ser235/236)
Specificity Traditional biochemical and lysate-based assays (e.g., western blot, immunoprecipitation, ELISA) have been integral in the analysis of individual signaling events, however they are limited in their ability to monitor the phosphorylation and subcellular localization of multiple proteins on a per cell basis. PathScan
Purification Phospho-Akt (Ser473) antibody detects endogenous levels of Akt only when phosphorylated at Ser473. Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody detects endogenous levels of p44 and p42 MAP kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases. Phospho-S6 ribosomal protein (Ser235/236) antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and Ser236.
Supplier Cell Signaling Technology
Dilution 8998,8997,Alexa Fluor
Catalog Number 8999

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