Monoclonal antibodies were produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase and Tyr1068 of human EGF receptor, or with a fusion protein containing the cytoplasmic domain of human EGF receptor.

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Category Category: Primary Antibodies
Publication 0
Application Primary Cocktail,Detection Cocktail,EGF Receptor,Phospho-EGF Receptor (Tyr1068),Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
Specificity The PathScan
Purification EGF receptor antibody detects endogenous levels of total EGF receptor protein and does not cross-react with other ErbB family members. Phospho-EGF receptor (Tyr1068) antibody detects endogenous EGF receptor only when phosphorylated at Tyr1068. This antibody may cross-react weakly with other tyrosine-phosphorylated proteins. Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody detects endogenous levels of p44 and p42 MAP kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases.
Supplier Cell Signaling Technology
Dilution 7972,7973,Alexa Fluor
Catalog Number 7967

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