Monoclonal antibodies were produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3, a synthetic peptide corresponding to residues surrounding Asp213 of human PARP, or with full-length chicken ?-tubulin purified from brain extracts.

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Category Category: Primary Antibodies
Publication 0
Application Primary Cocktail,Detection Cocktail,Phospho-Histone H3 (Ser10),Cleaved-PARP (Asp214),?-Tubulin
Specificity The PathScan
Purification ?-Tubulin antibody detects endogenous levels of total ?-tubulin protein. Phospho-Histone H3 (Ser10) antibody detects endogenous levels of histone H3 only when phosphorylated at Ser10. This antibody does not cross-react with other phosphorylated or acetylated histones. Cleaved-PARP (Asp214) detects endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. This antibody does not recognize full length PARP1 or other PARP isoforms.
Supplier Cell Signaling Technology
Dilution 7847,7843,Alexa Fluor
Catalog Number 7851

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