Phospho-MAPK Substrates (PXTP) (46G11) Rabbit mAb

The MAPK and CDK families of serine/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-3). MAPK phosphorylates substrates with the consensus sequence PX(S/T)P, and CDKs phosphorylate substrates containing the consensus sequence (S/T)PXR/K. Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline, motifs PXS*/T*P and/or S*PXR/K, for use in the study and discovery of new MAPK and CDK substrates (4,5).

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Category Category: Primary Antibodies
Publication 0
Application Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin),Flow Cytometry,Peptide ELISA (DELFIA)
Specificity Phospho-MAPK Substrates (PXT*P) (46G11) Rabbit mAb detects phospho-threonine in a PXT*P motif. The antibody is phospho-specific, and does not react with phospho-serine- or phospho-tyrosine-containing peptides/proteins. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Host Rabbit
Reactivity Human, All Species Expected
Purification Monoclonal antibody is produced by immunizing animals with synthetic phospho-MAPK/CDK substrate peptides .
Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100
Supplier Cell Signaling Technology
Dilution 1:1000,1:50,1:600,1:100,1:1000
Catalog Number 4391
ELISA Sensitivity Endogenous