NF-?B p65 (E498) Antibody

Transcription factors of the nuclear factor ?B (NF-?B)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-?B1 (p105/p50), and NF-?B2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-?B is sequestered in the cytoplasm by I?B inhibitory proteins (3-5). NF-?B-activating agents can induce the phosphorylation of I?B proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-?B to enter the nucleus where it regulates gene expression (6-8). NIK and IKK? (IKK1) regulate the phosphorylation and processing of NF-?B2 (p100) to produce p52, which translocates to the nucleus (9-11).

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Category Category: Primary Antibodies
Publication 0
Application Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry),Flow Cytometry
Specificity NF-?B p65 (E498) Antibody detects endogenous levels of total NF-?B p65/RelA protein. It does not cross-react with other NF-?B/Rel family members.
Host Rabbit
Reactivity Human, Mouse, Rat, Hamster, Monkey, Monkey, Bovine, Dog, Pig, Guinea Pig
Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu498 of human NF-?B p65/RelA protein. Antibodies are purified by protein A and peptide affinity chromatography.
Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100
Supplier Cell Signaling Technology
MW 65
Dilution 1:1000,1:50,1:200,1:100,1:100
Catalog Number 3987
ELISA Sensitivity Endogenous