AFP (3H8) Mouse mAb

Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and ?-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and ?-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma (3).

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Category Category: Primary Antibodies
Publication 0
Application Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry
Specificity AFP (3H8) Mouse mAb detects endogenous levels of total AFP protein.
Host Mouse
Reactivity Human
Purification Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human AFP.
Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100
Supplier Cell Signaling Technology
MW 70
Dilution 1:1000,1:100,1:200
Catalog Number 3903
Size 100
ELISA Sensitivity Endogenous