Anti-Glu-Glu Epitope Tag Affinity Matrix (Previously Covance catalog# AFC-115P)

It is extremely specific and recognizes either six amino acid sequence, EYMPME, or EFMPME. This antibody was purified using protein-G chromatography and was subsequently immobilized onto a Sepharose™ Fast Flow matrix.

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Price $540.00
Publication 0
Application Purification, IP
Host Mouse IgG1
Reactivity EYMPME and EFMPME Tags
Purification The antibody was purified by affinity chromatography.
immunogen The Glu-Glu monoclonal antibody was raised against the peptide sequence CEEEEYMPME derived from the polyoma virus medium T antigen
Application Note This affinity matrix can be used for immunopurification of Glu-Glu epitope tagged fusion proteins from crude starting material. On an analytical scale, it can also be used for immunoprecipitating Glu-Glu epitope tagged proteins.Sepharose is a trademark of  GE Life Sciences
Form Purified IgG immobilized on Sepharose™ Fast Flow beads (in PBS + 0.03% Thimerosal).
Storage Store between 2-8°C. The matrix may be re-used several times. To strip column after use, wash with several bead volumes of 0.1 M glycine pH 2.8 followed immediately by PBS containing 0.3% Thimerosal or 1mM Sodium Azide as preservative.
Supplier Biolegend
Isotype Mouse IgG1
Dilution Each lot of this antibody is quality control tested by immunoprecipitation.The optimal buffers and matrix concentration should be determined for each specific assay condition.Binding: Tagged protein will bind to matrix in common physiologic buffers with pH in the range of 6.0-7.5, salt from 50-500 mM and in the presence of reasonable levels of detergent. Excess reducing agent should be avoided as the disulfide bridges holding antibody heavy and light chains may be compromised. BioLegend tests Affinity Matrix using an equilibration/binding buffer containing:• 100 mM Tris-HCl (pH 7.5)• 150 mM NaCl• 0.1% Tween 20• 0.5% BSA• 1 mM beta-mercaptoethanolWashing: After binding, washes with several bead volumes of buffer are recommended. Such buffer may contain increased salt, altered pH, etc. as determined empirically to remove un-tagged proteins.Elution: Several options are available for elution.1. SDS gel loading buffer may be applied directly to the beads in order to display all bound protein on a polyacrylamide gel/western. Note that gel loading buffer containing reducing agent will also release some antibody heavy and light chains (approx 25 and 50kD, respectively).2. Competitive elution with epitope peptide. For epitope tag affinity matrices, prepare an elution buffer with epitope tag peptide at 400 ug/mL in 50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM EDTA (pH 8.0).3. Chemical Elution. Elution by pH or chaotropic salts is also possible. For elution by pH, either 0.1 M glycine pH 2.8 or 40 mM diethyl-amine pH 11.0 may be used.
Catalog Number 900301
Concentration Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Size 1 ml
Clone Glu-Glu

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