Anti-mCherry Nanobody Affinity Gel

mCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. It is the most widely used photostable protein in the class of red-shifted fluorescent proteins. mCherry derives from proteins initially isolated from Cnidarians. Nanobodies are single-domain antibodies. They are usually derived from camelids such as llamas, which make a unique subset of immunoglobulins that consist of heavy-chain homodimers without light chains. Their variable region (VHH) is the smallest antigen-binding single polypeptide chain found in any natural antibody. Nanobodies are recombinant antigen-binding domains derived from these VHH regions. Unlike monoclonal antibodies, they can readily be produced in large amounts in bacterial expression systems. Moreover, nanobodies are usually stable; they can bind antigens with affinities in the nanomolar range and are smaller in size (approximately 15 kD) than other antibody constructs. Experimentally, nanobodies have been applied in WB, IF, and IP. In IP/WB applications, nanobodies can avoid IgG heavy/light chain issues that occur when using regular antibodies.

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Price $425.00
Publication 5
Application IP
Host Derived from IgGVHH.
Reactivity Discosoma mCherry
Purification The antibody was purified by affinity chromatography.
immunogen Recombinant mCherry.
Form 50% anti-mCherry nanobody (clone LaM-4) conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
Storage Upon receipt, store between 2°C and 8°C. The unopened product is stable for one year upon arrival.
Supplier Biolegend
Isotype Derived from IgGVHH.
Dilution For immunoprecipitation, the suggested use of this reagent is 5 - 20 µl affinity gel for 100 µg lysate harvested from mCherry overexpressed cells.
Catalog Number 689502
Alternative Names DSRED
Size 1 ml
Clone LaM-4