Direct-Blot™ HRP anti-Cytokeratin (pan reactive) Antibody

Cytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus.

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Category Antibodies
Price $290.00
Publication 0
Application WB
Host Mouse IgG1, κ
Reactivity Recognizes Humancytokeratin 4, 5, 6, 8, 10, 13, and 18.
Purification The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
immunogen Keratin enriched fraction from human epidermoid carcinoma.
Application Note The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), and immunocytochemistry.
Form This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Storage Upon receipt, the antibody solution should be stored undiluted at -20°C.
Supplier Biolegend
Isotype Mouse IgG1, κ
Dilution Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest.
Catalog Number 628609
Concentration Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Alternative Names Cytokeratin, Keratin
Size 100 µl (20 Western blots)
Clone C-11