Alexa Fluor® 488 anti-Cytokeratin (pan reactive) Antibody

Cytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus.

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Category Antibodies
Price $295.00
Publication 0
Application IF, IHC_P
Host Mouse IgG1, κ
Reactivity Recognizes Humancytokeratin 4, 5, 6, 8, 10, 13, and 18.
Purification The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
immunogen Keratin enriched fraction from human epidermoid carcinoma.
Application Note The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), and immunocytochemistry.
Form Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Supplier Biolegend
Isotype Mouse IgG1, κ
Dilution Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 1.25 - 5.0 μg/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Catalog Number 628608
Concentration 0.5 mg/ml
Alternative Names Cytokeratin, Keratin
Size 100 µg
Clone C-11
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