Purified anti-Cytokeratin (pan reactive) Antibody

Cytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus.

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Category Antibodies
Price $85.00;$185.00
Publication 0
Application WB, IF, IP, IHC, ICC
Host Mouse IgG1, κ
Reactivity Recognizes Humancytokeratin 4, 5, 6, 8, 10, 13, and 18.
Purification The antibody was purified by affinity chromatography.
immunogen Keratin enriched fraction from human epidermoid carcinoma.
Application Note The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), and immunocytochemistry.
Form This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Storage The antibody solution should be stored undiluted between 2°C and 8°C.
Supplier Biolegend
Isotype Mouse IgG1, κ
Dilution Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1-4 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Catalog Number 628601
Concentration 0.5 mg/ml
Alternative Names Cytokeratin, Keratin
Size 25 µg;100 µg
Clone C-11
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