Purified anti-Cytokeratin 18 Antibody

Keratin 18 (also know as cytokeratin 18) is a 48 kD member of the Type I intermediate filament family, that forms heterotetramers. Keratin 18 is widely expressed in normal single layer epithelial tissues (liver, pancreas) and is expressed at high levels in some tumor tissues. Keratin 18 and filament partner keratin 8 are the most common members of intermediate filament family. Mutations in keratin 18 have been linked to cryptogenic cirrhosis. This protein is induced by retinoic acid, estrogen (breast cancer cells), and the Ras-signaling pathway (Ha-Ras, activated Src, Lck, Raf). Keratin 18 can be modified by phosphorylation, glycosylation, and ubiquitination. The active heterotetramer consists of two type I and two type II keratins (type I is acidic 40-55 kD, type II is neutral to basic 56-70 kD). Keratin 18 has been shown to associate with keratin 8, Mrj (a DnaJ/HSP40 family protein), and Hsp70. The Poly6172 antibody recognizes human and mouse keratin 18 and is useful for Western blotting.

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Category Antibodies
Price $75.00;$225.00
Publication 4
Application WB
Host Rabbit Polyclonal IgG
Reactivity Human, weakly cross-reacts with mouse
Purification The antibody was purified by antigen-affinity chromatography.
immunogen Recombinant (partial), C-terminal
Form This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Storage Upon receipt, store frozen at -20°C.
Supplier Biolegend
Isotype Rabbit Polyclonal IgG
Dilution Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Catalog Number 617201
Concentration Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Alternative Names Keratin 18, Keratin type I cytoskeletal 18
Size 50 µl (5 Western blots);200 µl (20 Western blots)
Clone Poly6172