Ultra-LEAF™ Purified Human IgG4 Isotype Ctrl Antibody

Immunoglobulin G4, or IgG4, is a type of antibody. Humans express four types of IgGs, IgG1, IgG2, IgG3, and IgG4. Antibodies in mammals are complex proteins, composed of four independent amino acid chains. The molecule is formed by two identical heavy chains and two identical light chains. It has two binding sites, and it is produced by B cells. When the immunoglobulins are expressed in the surface of the B cell it forms the B cell receptor. Antibodies are very important players of humoral immunity and IgG4 constitutes the fourth and least abundant IgG in human blood.

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Category Isotype control
Price $300.00
Publication 0
Application ELISA, FC
Host Human IgG4, κ
Reactivity Hapten molecule not expressed in mammalian cells
Purification The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Application Note The human IgG4 monoclonal antibody was produced as a recombinant protein in mycoplasma free CHO cells under serum-free conditions. This antibody reacts against a hapten that is not expressed in mammalian cells. It can be used as an isotype control when evaluating fully human, humanized, or chimeric antibodies. The human IgG4 monoclonal antibody is suitable as an isotype-matched control for ELISA, immunoblotting, FACS, IHC, and functional studies. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. 403402) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Form 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is
Storage The antibody solution should be stored at -80°C. It can be stored under sterile conditions for one month between 2°C and 8°C, or six months at -70°C to -80°C in a manual defrost freezer. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Supplier Biolegend
Isotype Human IgG4, κ
Dilution Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Catalog Number 403402
Concentration 1.0 mg/ml
Alternative Names IGHG4, immunoglobulin heavy chain constant region gamma 4
Size 500 µg
Clone ET904

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