Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl Antibody

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

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Category Isotype control
Price $95.00;$325.00;$625.00;$1,750.00
Publication 0
Application FC, ICFC, WB, IP, ICC, IF, IHC, FA
Host Rat IgG2a, κ
Reactivity KLH
Purification The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
immunogen Trinitrophenol + KLH
Application Note The RTK2758 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 400516) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400544) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Form 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is
Storage The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Supplier Biolegend
Isotype Rat IgG2a, κ
Dilution Each lot of this rat IgG2a, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Catalog Number 400543
Concentration For 5 mg and 25 mg the antibody is bottled at the concentration indicated on the vial, typically between 1 mg/ml and 3 mg/ml. For smaller sizes the concentration is 1.0 mg/ml.
Size 100 µg;1 mg;5 mg;25 mg
Clone RTK2758

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