LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl Antibody

The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

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Category Isotype control
Price $30.00;$105.00
Publication 0
Application FC, WB, IP, ICFC, ICC, IF, IHC, FA
Host Mouse IgG2a, κ
Purification The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Application Note The MOPC-173 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 400224) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400264) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Form 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is
Storage The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Supplier Biolegend
Isotype Mouse IgG2a, κ
Dilution Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Catalog Number 400223
Concentration 1.0 mg/ml
Size 50 µg;500 µg
Clone MOPC-173

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