Purified anti-human HLA-DR (MaxPar® Ready) Antibody

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.

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Category Antibodies
Price $120.00
Publication 7
Application FC, CyTOF
Host Mouse IgG2a, κ
Reactivity Human, Cross-Reactivity: African Green, Baboon, Monkey, Common Marmoset, Cotton-topped Tamarin, Monkey, Monkey, Monkey, Squirrel Monkey, Canine(Canine)17, 18
Purification The antibody was purified by affinity chromatography.
Application Note The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRα which depends on the correct folding of the αβ heterodimer.19Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, and immunohistochemical staining of acetone-fixed frozen sections4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 307612). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 307648) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Form Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Storage The antibody solution should be stored undiluted between 2°C and 8°C.
Supplier Biolegend
Isotype Mouse IgG2a, κ
Dilution This product is suitable for use with the MaxPar® Metal Labeling Kits. The product is formulated so that the buffer exchange step is not required (steps 7, 8, 9, and 11 in the MaxPar® antibody labeling protocol). Just add 100 µl of this antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter, as described in step 12, and continue with the protocol.
Catalog Number 307651
Concentration 1.0 mg/ml
Alternative Names Major Histocompatibility Class II, MHC class II
Size 100 µg
Clone L243