Purified Streptavidin

Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.

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Price $35.00
Publication 5
Application FC, ICFC, ELISA_Capture, ELISA_Detection, IF, IHC_F, IHC_P, IP, WB
Form Phosphate-buffered solution containing 0.09% sodium azide.
Storage Store between 2°C and 8°C.
Supplier Biolegend
Dilution To coat one 96-well plate dilute 24 µl of Purified Streptavidin into 11.976ml of 1X Coating Buffer (Cat# 421701, Coating Buffer 5X concentrate). Final concentration in the coated plate is 2 µg/ml. This recommendation is to perform the protocol for HLA class I ELISA to evaluate Flex-T™ peptide exchange. For other applications the reagent needs to be optimized.
Catalog Number 280302
Concentration 1.0 mg/ml
Size 250 µg

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