Mouse MDSC Flow Cocktail 2 with Isotype Ctrl

This mouse MDSC Flow Cocktail 2 is composed of CD11b, Ly-6C, and Ly-6G antibodies. MDSCs are a heterogenous population of cells that can affect T cell function. Two different subsets of MDSC exist: CD11b+Ly-6G+Ly-6Clow (granulocytic MDSC) and CD11b+Ly-6G-Ly-6Chi (monocytic MDSC). These cells have been identified in a variety of pathological conditions including cancer and autoimmune disease.CD11b: CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.Ly-6G: Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.Ly-6C: Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

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Category Isotype control
Price $295.00
Publication 10
Application FC
Host Rat IgG2a, Rat IgG2b, Rat IgG2c, κ
Reactivity Mouse
Purification This reagent is a multicolor cocktail of PE conjugated CD11b (clone M1/70), FITC conjugated Ly-6G (clone 1A8) and APC conjugated Ly-6C (clone HK1.4) at optimal concentration for three-color flow cytometric analysis.
Application Note Materials provided: 1. Mouse MDSC Flow Cocktail (containing CD11b PE/Ly-6G FITC/Ly-6C APC), 25 tests 2. Mouse MDSC Isotype Control Cocktail (containing rat IgG2b PE/rat IgG2a FITC/rat IgG2c APC), 25 testsMaterials not provided: Cell Staining Buffer (Cat. No. 420201) Single color compensation controls
Form Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Supplier Biolegend
Isotype Rat IgG2a, Rat IgG2b, Rat IgG2c, κ
Dilution Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Catalog Number 147003
Concentration Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Alternative Names Myeloid derived suppressor cells flow kit, CD11b/Ly6G/Ly6C
Size 25 tests
Clone M1/70/1A8/HK1.4