Alexa Fluor® 647 anti-mouse H-2Kb Antibody

The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

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Category Antibodies
Price $85.00;$195.00
Publication 3
Application FC, IF, IHC
Host Mouse (BALB/c) IgG2a, κ
Reactivity Mouseb haplotype, Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v)
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
immunogen C57BL mouse splenocytes
Application Note Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.
Form Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Supplier Biolegend
Isotype Mouse (BALB/c) IgG2a, κ
Dilution Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Catalog Number 116511
Concentration 0.5 mg/ml
Alternative Names MHC class I
Size 25 µg;100 µg
Clone AF6-88.5
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