Purified anti-mouse H-2Kb Antibody

The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

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Category Antibodies
Price $65.00;$215.00
Publication 3
Application FC, IHC, IP
Host Mouse (BALB/c) IgG2a, κ
Reactivity Mouseb haplotype, Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v)
Purification The antibody was purified by affinity chromatography.
immunogen C57BL mouse splenocytes
Application Note Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.
Form Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted between 2°C and 8°C.
Supplier Biolegend
Isotype Mouse (BALB/c) IgG2a, κ
Dilution Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Catalog Number 116501
Concentration 0.5 mg/ml
Alternative Names MHC class I
Size 50 µg;500 µg
Clone AF6-88.5