Alexa Fluor® 647 anti-mouse H-2Kd/H-2Dd Antibody

The 34-1-2S antibody reacts with the H-2Kd/H-2Dd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kd/H-2Dd haplotype. H-2Kd/H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.

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Category Antibodies
Price $195.00
Publication 3
Application IF, IHC, FC
Host Mouse (C3H) IgG2a, κ
Reactivity Cross-reacts with H-2Kb, s, r, q, p
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
immunogen BDF Splenocytes
Application Note Additional reported applications (for the relevant formats) include: immunoprecipitation2, complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.
Form Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Supplier Biolegend
Isotype Mouse (C3H) IgG2a, κ
Dilution Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Catalog Number 114712
Concentration 0.5 mg/ml
Alternative Names MHC class I
Size 100 µg
Clone 34-1-2S