Alexa Fluor® 647 anti-mouse H-2Kb/H-2Db Antibody

The 28-8-6 antibody reacts with the H-2Kb and H-2Db MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kb/H-2Db haplotype. H-2Kb/H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2Dd MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.

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Category Antibodies
Price $195.00
Publication 3
Application FC, IF, IHC
Host Mouse (C3H) IgG2a, κ
Reactivity Mouseb haplotype, Cross-reacts with H-2Dd MHC class I alloantigen. Does not react with other haplotypes (e.g., f, k, p, q, r, s)
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
immunogen C3H.SW mouse splenocytes
Application Note Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.
Form Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Supplier Biolegend
Isotype Mouse (C3H) IgG2a, κ
Dilution Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Catalog Number 114612
Concentration 0.5 mg/ml
Alternative Names MHC class I
Size 100 µg
Clone 28-8-6
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