Alexa Fluor® 647 anti-mouse H-2D d Antibody

The 34-2-12 antibody recognizes α3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype. H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-2-12 antibody cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported. Clone 34-2-12 recognizes the α3 domain of Dd.

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Category Antibodies
Price $195.00
Publication 3
Application FC, IF, IHC
Host Mouse (C3H/HeJ) IgG2a, κ
Reactivity Moused haplotype, Cross-reacts with the C3H.LG/Ckc. Does not cross react with other haplotypes (e.g., b, f, k, p, q, r, s).
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
immunogen (C57BL/6 x DBA/2)F1 mouse splenocytes
Application Note Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2,3, blocking of cell adhesion4,5, and immunohistochemical staining of acetone-fixed frozen sections6.
Form Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Supplier Biolegend
Isotype Mouse (C3H/HeJ) IgG2a, κ
Dilution Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Catalog Number 110612
Concentration 0.5 mg/ml
Alternative Names MHC class I
Size 100 µg
Clone 34-2-12