LEAF™ Purified anti-mouse NK-1.1 Antibody

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

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Category Antibodies
Price $45.00;$145.00
Publication 4
Application FC, IP, CMCD, Depletion, Lysis, Block, Activ, IHC, IF, CyTOF
Host Mouse IgG2a, κ
Reactivity Mouse
Purification The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
immunogen NK-1+ cells from mouse spleen and bone marrow
Application Note Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, and immunofluorescence microscopy11. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 108712).
Form 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is
Storage The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Supplier Biolegend
Isotype Mouse IgG2a, κ
Dilution Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Catalog Number 108711
Concentration 1.0 mg/ml
Alternative Names NKR-P1C, NKR-P1B, Ly-55, CD161b, CD161c
Size 50 µg;500 µg
Clone PK136
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