LEAF™ Purified anti-mouse/human CD44 Antibody

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

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Category Antibodies
Price $80.00;$260.00;$450.00
Publication 6
Application FC, ELISA, IHC, IP, CMCD, Stim, CyTOF
Host Rat IgG2b, κ
Reactivity Mouse, Human, Cross-Reactivity: Baboon, Monkey, Monkey, Monkey, Squirrel Monkey, Horse(Equine), Felinetle (Bovine, Bovine), Swine (Pig, Pig), Canine(Canine), Feline(Feline)
Purification The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
immunogen Dexamethasone-induced myeloid leukemia M1 cells
Application Note Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103014). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 103046) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Form 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is
Storage The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Supplier Biolegend
Isotype Rat IgG2b, κ
Dilution Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Catalog Number 103013
Concentration 1.0 mg/ml
Alternative Names Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Size 50 µg;500 µg;1 mg
Clone IM7
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