LEAF™ Purified anti-mouse CD3ε Antibody

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

  • Product Detail
  • All Images
Category Antibodies
Price $25.00;$105.00;$195.00
Publication 3
Application FC, IHC, IP, WB, CMCD, Block, Apop, Depletion, Activ, IF
Host Armenian Hamster IgG
Reactivity Mouse
Purification The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
immunogen H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Application Note The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletion. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Form 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is
Storage The CD3ε antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Supplier Biolegend
Isotype Armenian Hamster IgG
Dilution Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. This product is included in our Activation Bundles. Learn more...
Catalog Number 100313
Concentration 1.0 mg/ml
Alternative Names CD3ε, T3, CD3
Size 50 µg;500 µg;1 mg
Clone 145-2C11
Feedback