Ab00798-23.0 Anti-Hemagglutinin 5 [H5.3]

Recombinant monoclonal antibody to Hemagglutinin 5. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma H5.3.

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Category Antibodies
Price 255
Publication 1
Application Neutralize
Specificity H5.3 binds to H5 at the head domain at an epitope which involves the 190 loop. The epitope was determined to be LGVSSA (aa 134-139) and YQGKSS (aa 142-147) of the head domain by DXMS (deuterium exchange mass spectrometry). This antibody shows reactivity with the wild-type H5N1 field strains VN/1203 (EC50 ~ 17 ng/ml), HK/156 (EC50 ~ 18 ng/ml), Anhui (EC50 ~ 2.8 ug/ml) and Indo (EC50 ~ 10 ug/ml). HA is a viral glycoprotein initially assembled as a trimer on the viral envelope as the preprotein HA0. It is subsequently cleaved by host proteases.
Reactivity Viral virus H5N1 (VN/1203,HK/156,Anhui,Indo)
Clonality Monoclonal
Purification Protein A affinity purified
immunogen H5.3 was generated from hybridomas created by fusing PBMCs (peripheral blood mononuclear cells) from human donors who had been previously received an A/Vietnam/1203/2004 H5N1 subunit vaccine with myeloma cells. The hybridoma cells were screened for those showing binding affinity for H5 HA.
Application Note H5.3 binds to H5 where it is able to block the receptor binding site on HA by inserting its CDR3 into the site (IC50 ~ 20 ng/ml). H5.3 can neutralize wild-type VN/1203, rdt VN/1203 K and VN/1203 F pseudovirions, all with IC50s of 5 ng/ml. This antibody also has potent HI (hemagglutination inhibition) activity (80 ng/ml).
Form Supplied in: Bulk size: PBS only. Standard size: PBS with 0.02% Proclin 300.
Storage Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Supplier absoluteantibody
Isotype Rabbit IgG, lambda
Catalog Number Ab00798-23.0
Concentration See vial label.
Conjugation Unconjugated
Size 200 µg
Uniport Q5EP31
Clone H5.3

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