Rat anti mouse F4/80 antibody, clone Cl:A3-1, recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen (Hume, et al., 1984). F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node (Gordon, et al., 1994.). The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells (Lin, et al., 2005) Clone Cl:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (Warschkau & Kiderlen, 1999.).An anti-idiotypic CI:A31antibody, clone 17867, is also available from AbD Serotec which is designed as a control antibody for use in conjunction with clone Cl:A3-1. This product, HCA154, specifically binds to and blocks this F4/80 antibody.

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Category Antibodies
Price £312
Publication 3
Application IHC-Fr, WB, IHC-P, IP, FC, IHC-R, RIA, EM, IF/ICC
Target F4/80
Host Rat
Reactivity Mouse
Clonality Monoclonal
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Form Purified IgG - liquid
Storage Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Supplier AbDSerotec
Isotype IgG2b
Dilution Western Blotting-
Catalog Number MCA497G
Conjugation Unconjugated
Size 0.5 mg
Uniport Q61549
Clone Cl:A3-1